DNA Extraction:Article Title: An ancient anthozoan protein reveals an alternative evolutionary path of antiviral signaling
Article Snippet: .. Samples were dried at 50 °C for 10 min, after which DNA extraction buffer (10 mM Tris pH 8, 1 mM EDTA pH 8, 25 mM NaCl, and 200 μg/ml Proteinase K; Thermo Fisher Scientific, USA) was added. .. Samples were incubated for 2 h at 50 °C, followed by 5 min at 96 °C to inactivate Proteinase K. Mutation analysis was performed using high-resolution melting (HRM) on a Magnetic Induction Cycler qPCR instrument (Bio Molecular Systems, Australia) to detect indels.
Virus:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
Recombinant:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
Protease Inhibitor:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
Article Title: UFMylation anchors splicing factors at the ER to reprogram nuclear splicing
Article Snippet: .. Briefly, 1.5–2 g seedlings were homogenized in liquid nitrogen and resuspended in 6 mL of microsome extraction buffer (MEB; 100 mM Tris-HCl pH 8.0, 5 mM EGTA, 15 mM MgCl 2 , 2 μ M DTT, 0.3 M sucrose (Sigma-Aldrich), 20 U mL −1 SUPERase·In RNase Inhibitor (Thermo Fisher Scientific), 50 μ mL −1 cycloheximide (Sigma-Aldrich), and 1× Protease inhibitor cocktail). ..
Article Title: Inflammatory cytokines induce new cancer dependencies.
Article Snippet: Collins K. Cheruiyot, Sarah Y. Kim, Juan Dubrot , Sarah K. Lane-Reticker, Alex Miranda, Ashwin V. Kammula , Jonathan J. Perera , Peter P. Du , Cun Lan Chuong, Rachel A. Fetterman, Nelson H. Knudsen , Aiping Jiang , Juliette S. M. T. Suermondt, Lomax F. Pass, Cong Fu, Meng-Ju Wu , Lei Shi , Seth Anderson , Audrey J. Muscato , Omar I. Avila , Ian C. Kohnle, Emily A. Kessler , Hans W. Pope, Sarah G. Noel , Kira E. Olander , Jooho Chung , Kayla J. Colvin, Nabeel Bardeesy, Kathleen B. Yates & Robert T. Manguso
Lysis:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
Article Title: Inflammatory cytokines induce new cancer dependencies.
Article Snippet: Collins K. Cheruiyot, Sarah Y. Kim, Juan Dubrot , Sarah K. Lane-Reticker, Alex Miranda, Ashwin V. Kammula , Jonathan J. Perera , Peter P. Du , Cun Lan Chuong, Rachel A. Fetterman, Nelson H. Knudsen , Aiping Jiang , Juliette S. M. T. Suermondt, Lomax F. Pass, Cong Fu, Meng-Ju Wu , Lei Shi , Seth Anderson , Audrey J. Muscato , Omar I. Avila , Ian C. Kohnle, Emily A. Kessler , Hans W. Pope, Sarah G. Noel , Kira E. Olander , Jooho Chung , Kayla J. Colvin, Nabeel Bardeesy, Kathleen B. Yates & Robert T. Manguso
Staining:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
Extraction:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
Article Title: UFMylation anchors splicing factors at the ER to reprogram nuclear splicing
Article Snippet: .. Briefly, 1.5–2 g seedlings were homogenized in liquid nitrogen and resuspended in 6 mL of microsome extraction buffer (MEB; 100 mM Tris-HCl pH 8.0, 5 mM EGTA, 15 mM MgCl 2 , 2 μ M DTT, 0.3 M sucrose (Sigma-Aldrich), 20 U mL −1 SUPERase·In RNase Inhibitor (Thermo Fisher Scientific), 50 μ mL −1 cycloheximide (Sigma-Aldrich), and 1× Protease inhibitor cocktail). ..
Article Title: Inflammatory cytokines induce new cancer dependencies.
Article Snippet: Collins K. Cheruiyot, Sarah Y. Kim, Juan Dubrot , Sarah K. Lane-Reticker, Alex Miranda, Ashwin V. Kammula , Jonathan J. Perera , Peter P. Du , Cun Lan Chuong, Rachel A. Fetterman, Nelson H. Knudsen , Aiping Jiang , Juliette S. M. T. Suermondt, Lomax F. Pass, Cong Fu, Meng-Ju Wu , Lei Shi , Seth Anderson , Audrey J. Muscato , Omar I. Avila , Ian C. Kohnle, Emily A. Kessler , Hans W. Pope, Sarah G. Noel , Kira E. Olander , Jooho Chung , Kayla J. Colvin, Nabeel Bardeesy, Kathleen B. Yates & Robert T. Manguso
Reverse Transcription:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
SYBR Green Assay:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
DC Protein Assay:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
Software:Article Title: SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.
Article Snippet: Organometallics 29, 2176–2179. https://doi.org/10.1021/om100106e. ll OPEN ACCESSBrief communication Cell Chemical Biology 33, 1–9, July 16, 2026 9 STAR★METHODS .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains See Table S2 for bacterial strains used NA NA Chemicals, peptides, and recombinant proteins Mueller Hinton Agar Millipore Cat#: 70191 Mueller Hinton Broth Millipore Cat#: 70192 OXF-077 Bradbury et al.8 NA PK-150 Le et al.,21 Sigma-Aldrich Cat#: SBR00043 EDTA-free Protease Inhibitor Cocktail Roche Cat#: 04693159001 Pathogen Lysis Tubes S QIAGEN Cat#: 19091 AzTB 2 B Scientific Cat#: CCT-1048 AzT Sigma-Aldrich Cat#: 762024 4 × NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat#: NP0007 NuPAGE 12% Bis-Tris Mini Protein Gels Thermo Fisher Scientific Cat#: NP0342BOX QuickBlue Protein Stain LuBio Cat#: LU001000-LBO NeutrAvidin Agarose Resin Thermo Fisher Scientific Cat#: 29200 LysC Wako Cat#: 129-02541 Trypsin Promega Cat#: V5111 C18 extraction disks (for STAGETips) Empore Cat#: 2215 RNAprotect QIAGEN Cat#: 76506 RNeasy Mini Kit QIAGEN Cat#: 74104 RNase-Free DNAse Set QIAGEN Cat#: 79254 QuantiTect Reverse Transcription Kit QIAGEN Cat#: 205311 SYBR Green BioRad Cat#: 1725271 Critical commercial assays DC Protein Assay Kit BioRad Cat#: 5000111 Deposited data Chemoproteomics This paper PRIDE: PXD071059 https://www.ebi.ac.uk/pride/archive/ projects/PXD071059 Oligonucleotides recA forward (5′-AAGTACGTCGTGCAGA-3′) Cirz et al.,23 Merck Easy Oligos NA recA reverse (5′-TGACCCATTCGTTCGC-3′) Cirz et al.,23 Merck Easy Oligos NA rho forward (5′-GAAGCTGCTGAAGTCG-3′) Cirz et al.,23 Merck Easy Oligos NA rho reverse (5′-CGTCCATACGTGAACCC-3′′) Cirz et al.,23 Merck Easy Oligos NA Software and algorithms GraphPad Prism Dotmatics http://www.graphpad.com/scientific-software/prism/ Emperia Studio LI-COR https://www.licorbio.com/bio/empiria-studio CFX Maestro BioRad https://www.bio-rad.com/en-uk/sku/12013758-cfx-maestro- software-2-3-for-windows-pc Proteome Discoverer Thermo Fisher Scientific https://www.thermofisher.com/uk/en/home/industrial/mass- spectrometry/liquid-chromatography-mass-spectrometry-lcms/lc-ms-software/multi-omics-data-analysis/proteomediscoverer-software.html Perseus Tyanova et al.37 https://maxquant.net/perseus/ ll OPEN ACCESS Brief communication e1 Cell Chemical Biology 33, 1–9.e1–e7, July 16, 2026 .. Bacterial strains and culture conditions Bacterial strains, S. aureus JE2 and RN1 and derivatives outlined in Table S2, were revived from a frozen stock as an overnight culture grown on non-selective Mueller Hinton Agar (MHA, Millipore, UK) supplemented with associated antibiotics at 37 ◦C for 17 h. Overnight cultures were grown in Mueller Hinton Broth (MHB, Millipore, UK) supplemented with associated antibiotics to late stationary phase at 37 ◦C and 180 RPM in a shaking incubator (SI-200, Cole Parmer, UK).
other:
Flow Cytometry:Article Title: Gut-derived, inflammatory ILC2s disseminate type 2 immunity to remote lung in food allergen-challenged mice.
Article Snippet: 15 Dissemination of type 2 immunity along skin-gut-lung axes contributes to allergic march 16 progressions in atopic individuals, yet the underlying mechanisms remain incompletely 17 understood.. In mice, gastrointestinal helminth infections can mobilize a circulating pool 18 of gut-derived, inflammatory type 2 innate lymphoid cells (iILC2s), which in turn 19 establish an early type 2 immune response in remote lung.. Here, using the model 20 allergen ovalbumin (OVA), we demonstrate duodenum-restricted expansion of iILC2s is 21 similarly elicited in response to an ingested food allergen.
RNA Extraction:Article Title: Gut-derived, inflammatory ILC2s disseminate type 2 immunity to remote lung in food allergen-challenged mice.
Article Snippet: 15 Dissemination of type 2 immunity along skin-gut-lung axes contributes to allergic march 16 progressions in atopic individuals, yet the underlying mechanisms remain incompletely 17 understood.. In mice, gastrointestinal helminth infections can mobilize a circulating pool 18 of gut-derived, inflammatory type 2 innate lymphoid cells (iILC2s), which in turn 19 establish an early type 2 immune response in remote lung.. Here, using the model 20 allergen ovalbumin (OVA), we demonstrate duodenum-restricted expansion of iILC2s is 21 similarly elicited in response to an ingested food allergen.
Incubation:Article Title: Gut-derived, inflammatory ILC2s disseminate type 2 immunity to remote lung in food allergen-challenged mice.
Article Snippet: 15 Dissemination of type 2 immunity along skin-gut-lung axes contributes to allergic march 16 progressions in atopic individuals, yet the underlying mechanisms remain incompletely 17 understood.. In mice, gastrointestinal helminth infections can mobilize a circulating pool 18 of gut-derived, inflammatory type 2 innate lymphoid cells (iILC2s), which in turn 19 establish an early type 2 immune response in remote lung.. Here, using the model 20 allergen ovalbumin (OVA), we demonstrate duodenum-restricted expansion of iILC2s is 21 similarly elicited in response to an ingested food allergen.
Modification:Article Title: Gut-derived, inflammatory ILC2s disseminate type 2 immunity to remote lung in food allergen-challenged mice.
Article Snippet: 15 Dissemination of type 2 immunity along skin-gut-lung axes contributes to allergic march 16 progressions in atopic individuals, yet the underlying mechanisms remain incompletely 17 understood.. In mice, gastrointestinal helminth infections can mobilize a circulating pool 18 of gut-derived, inflammatory type 2 innate lymphoid cells (iILC2s), which in turn 19 establish an early type 2 immune response in remote lung.. Here, using the model 20 allergen ovalbumin (OVA), we demonstrate duodenum-restricted expansion of iILC2s is 21 similarly elicited in response to an ingested food allergen.
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